The 2-Minute Rule for flow cytometers
The 2-Minute Rule for flow cytometers
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Information-enriched characterization of cell populations. Morphological info from photographs can include to the richness of flow cytometry details. For example, the figure exhibits an if not regular apoptosis assay applying Annexin V and PI, incorporating cell imaging to characterize cells in Each and every populace to expose morphologically distinct attributes. These insights could not are actually attained from multiplex staining alone.
Whether or not you’re a beginner or a specialist, check out our means to advance your analysis and abilities in flow cytometry.
Combine acoustic focusing technologies with large-parameter Evaluation for versatile spectral unmixing and conventional workflows.
Explore new insights into your experimental info by visualizing populations making use of mixed fluorescence and morphology details. Our program interface wizards permit downsampling, concatenation (merged data files) and dimensionality instruments which have been constructed in to the software for an intuitive, streamlined info Investigation working experience.
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Right here, a highly skilled consumer has gated singlets confidently. Soon after analyzing the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate contains more than four% aggregates.
Pair your Attune flow cytometer using an autosampler for greater workflow effectiveness, and add robotic integration for optimum throughput.
Our researchers will style panels for you as many times you would like—totally free, no merchandise purchase required.
Early indications of this contamination had to begin with been dismissed as particles. Backgating to find new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also allows you to use morphological options to find out interesting subpopulations that will not be clear from flow cytometry info by yourself.
People can check out function visuals in actual-time to substantiate sample top quality, enhance protocols, and document the morphology of every mobile population recognized for Assessment. Imaging seize also benefits from acoustic focusing, which really helps to situation the cells to make sure that a sharp, centered impression is acquired.
Sound waves help precise mobile alignment, leading to Remarkable info quality and ~10X faster sample flow fees.
We previously shown the strength of imaging Auto-T/Ramos mobile interactions. Let’s examine just the inhabitants of biggest fascination, the double beneficial occasions, To find out more. We could now use extended morphological parameters (listed here, circularity and skewness of intensity) to further more look at the characteristics of such populations and refine gating on these situations, increasing knowledge robustness.
Designed-in dimensionality reduction instruments enhanced resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to verify expected inhabitants resolution in lysed total human blood. Consumer can check out activities from Each individual cluster for Visible confirmation of cell phenotypes.
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Gating on these prolonged parameters https://dev-westudy.accedo.gr/members/nestskin65/activity/2430567/ permits you to quickly and correctly establish populations of desire and ensure gating method with little if any guide review. The Attune application offers usage of state-of-the-artwork machine Mastering analysis resources within an expertly built person interface, rendering it exceptionally simple to operate.
As the cell line experienced Formerly been analyzed on the Attune CytPix Flow Cytometer, the scientists went back again to the pictures and had been able to doc the microbial an infection at least five times previously.
The Attune CytPix Flow Cytometer enables buyers to visualize samples all through acquisition, verify the accuracy of gating tactics, and support effects with responsible documentation for publication needs.
Right here, we demonstrate that by gating methods working with impression-centered quantitative parameters can distinguish interacting cells from coincident gatherings to more precisely assess interacting cells.