The best Side of cytometer
The best Side of cytometer
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Details-enriched characterization of mobile populations. Morphological information and facts from photos can include for the richness of flow cytometry knowledge. By way of example, the determine reveals an in any other case conventional apoptosis assay making use of Annexin V and PI, introducing cell imaging to characterize cells in Each and every populace to expose morphologically unique functions. These insights could not have already been attained from multiplex staining by yourself.
As samples are acquired about the Attune CytPix Flow Cytometer, the superior-pace brightfield digital camera captures and stores illustrations or photos of detected occasions at a level nearly six,000 illustrations or photos for each second, with regards to the flow price and image sizing. For better versatility, Attune Cytometric Computer software permits you to change the picture capture frequency as essential and select a selected gate for picture seize, if required.
Blend acoustic focusing engineering with significant-parameter Evaluation for versatile spectral unmixing and standard workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing that has a higher-velocity brightfield digital camera for simultaneous significant throughput flow cytometry and higher resolution brightfield imaging.
"A picture isn't really quite really worth 1000 dots, however it genuinely helps you to see pictures. It is excellent to check out photos that correlate with staining. For example, AnnexinV-constructive cells which might be morphologically distinctive than healthful cells, or CD14+ monocytes that happen to be more substantial plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Furthermore, it can be illuminating to see what number of doublets slip as a result of conventional singlet gates, and great to validate the status of unpredicted double-positive occasions in my Assessment."
Pair your Attune flow cytometer with an autosampler for greater workflow performance, and increase robotic integration for optimum throughput.
Dependable graphic quality even at large throughput Acoustic concentrating and a higher-pace camera Blend to picture these T cells regularly at minimal or significant flow prices. Effortlessly regulate aim and digicam configurations to meet experimental requirements.
The Attune NxT cytometer may be the trustworthy workhorse instrument your lab can depend upon for dependable and trusted performance. The Attune CytPix model incorporates a significant-speed brightfield camera and AI-pushed impression Examination application, with modern updates such as machine learning applications and an intuitive user interface, enabling Improved details precision, accuracy, and a lot quicker insights into sample biology.
Consumers can check out celebration illustrations or photos in serious-time to substantiate sample quality, improve protocols, and history the morphology of every cell populace determined for Examination. Imaging capture also Gains from acoustic concentrating, which helps to situation the cells in order that a sharp, centered picture is attained.
Sound waves allow precise cell alignment, leading to Remarkable knowledge high quality and ~10X more quickly sample flow fees.
We Beforehand shown the power of imaging Vehicle-T/Ramos cell interactions. Allow’s have a look at just the populace of finest fascination, the double constructive events, to learn more. We can now use extended morphological parameters (listed here, circularity and skewness of depth) to further take a look at the options of such populations and refine gating on these functions, rising information robustness.
Leverage the strength of State-of-the-art technological innovation and innovation to accelerate your discoveries. Have confidence in from the Attune Flow Cytometer relatives to deliver the reliability, pace, and precision that set your perform aside.
No clean/no lyse leukocyte Evaluation with imaging. Cells ended up acquired from 24-hour-previous blood by dilution in one mM EDTA ( Qualified UX/UI enables consumers to educate AI types for expanded apps. (A) The “Train Product” feature delivers annotation tools and serious-time product schooling precision.
Gating on these extended parameters permits you to speedily and correctly recognize populations of fascination and confirm gating approach with little or no guide evaluate. The Attune program features entry to condition-of-the-artwork equipment Discovering Examination tools in an expertly intended consumer interface, which makes it extremely simple to operate.
Since the cell line had Beforehand been analyzed over the Attune CytPix Flow Cytometer, the scientists more info went back to the images and were being in the position to document the microbial infection no less than 5 days earlier.
The Attune CytPix Flow Cytometer enables users to visualize samples all through acquisition, confirm the precision of gating techniques, and guidance outcomes with reliable documentation for publication functions.
What seemed to be a homogeneous populace is in fact extra various—an insight that should be viewed as when interpreting benefits.